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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12599*  Cite 

Network of stress fibers in an MDCK cell. This transmission electron micrograph of a flat-embedded MDCK cell was taken 60-70nm from the interface between the cell and the coverslip on which it was grown and fixed. The cells were cultured on Thermanox® coverslips, then chemically fixed with glutaraldehyde and osmium tetroxide before being stained en bloc with uranyl acetate. The cells were infiltrated with Spurr's® resin, placed under a vacuum for fifteen minutes, and then baked at 70° C. The coverslips were peeled away from the hardened resin wafers, which were then cut and thin-sectioned with a diamond knife. The 65 nm sections were placed on grids and post-stained with uranyl acetate and lead citrate. They were imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV. Because the section was taken just inside the plasma membrane, many vesicles can be seen in this image, particularly in the lower left corner. A neighboring cell in the upper right is shown to underlap the main cell in this image. Scale bar = 2µm.

Biological Sources
NCBI Organism Classification
Canis lupus familiaris
Cell Type
epithelial cell
Cell Line
Cellular Component
stress fiber
Anna Baccei
Susan Lancelle
Mount Holyoke College
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
glutaraldehyde fixed tissue
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 1706px 0.0076µm
Y 1818px 0.0076µm