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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12413*  Cite 
Description

Localization of MIIB/A (head, actin-binding domain of MIIA (myosin IIA), and tail domain of MIIB (myosin IIB)) in MIIA-deficient CHO.K1 cells. This Video corresponds to Fig. 10 B and video 6 from CB 183:543-554 , 2008. Images of GFP-MIIA/B (green) and paxillin-mOrange (magenta) were collected every 4 s using TIRF microscopy with 10 frames/s shown. Images were acquired in an inverted microscope (model IX70;Olympus). For dual GFP- mRFP/mCherry/mOrange, a dual-emission filter (z488/543) was used. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies).

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
epithelial cell
Cell Line
myosin IIA deficient CHO-K1
Cellular Component
myosin IIA/B
paxillin
Biological Context
Biological Process
intracellular motility
adhesion formation
Attribution
Names
Miguel Vicente-Manzanares
Margaret A. Koach
Leanna Whitmore
Marcelo L. Lamers
Published
JCB 183:543-554 , 2008
Pubmed
18955554
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12413
Archival Resource Key (ARK)
ark:/b7295/w9cil12413
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mOrangeFP
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X —— 0.147µm
Y —— 0.147µm
Time 4 seconds