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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12407*  Cite 
Description

Kinetoplast and flagellum of a Leishmania promastigote cell. The image shows a longitudinal section through the anterior end of the cell, where the flagellum emerges from the flagellar pocket. The basal body of the flagellum is connected at its proximal end to the mitochondrial membranes and the kinetoplast. The kinetoplast contains the mitochondrial DNA (kDNA), organised in a network of circular DNA molecules, which forms a compact disk-shaped nucleoid that is visible in this section as an electron dense bar-shaped structure. Leishmania mexicana promastigote forms (WHO strain MNYC/BZ/62/M379) were grown in M199 culture medium. Cells were fixed with glutaraldehyde, post-fixed with osmium tetroxide, dehydrated in ethanol and embedded in epoxy resin. Thin sections (~90 nm) were viewed on a Philips Technai 12 transmission electron microscope operating at 80 kV. magnification 23000.

Biological Sources
NCBI Organism Classification
Leishmania mexicana
Cell Type
parasite
Cell Line
WHO strain MNYC/BZ/62/M379
Cellular Component
kinetoplast
flagellar pocket
pro-basal body
flagellum
mitochondrial outer membrane
mitochondrial DNA
Biological Context
Biological Process
promastigote form
Attribution
Names
Eva Gluenz
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12407
Archival Resource Key (ARK)
ark:/b7295/w9cil12407
Grouping This image is part of a group.
Imaging
Image Type
Bbi:00000265
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3241px 1.104nm
Y 1452px 1.104nm