Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12405^* Cite

Description

Longitudinal section through a Leishmania promastigote cell. Leishmania mexicana promastigote forms (WHO strain MNYC/BZ/62/M379) were grown in M199 culture medium. Cells were fixed with glutaraldehyde, post-fixed with osmium tetroxide, dehydrated in ethanol and embedded in epoxy resin. Thin sections (~90 nm) were viewed on a Philips Technai 12 transmission electron microscope operating at 80 kV. Magnification 13000X.

Biological Sources

NCBI Organism Classification: Leishmania mexicana
Cell Type: parasite
Cell Line: WHO strain MNYC/BZ/62/M379

Biological Context

Biological Process

Attribution

Names: Eva Gluenz

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12405
Archival Resource Key (ARK): ark:/b7295/w9cil12405

Grouping This image is part of a group.

Imaging

Image Type: Bbi:00000265
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide
Processing History: unprocessed raw data
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2177px	1.952nm
Y	3303px	1.952nm