Alternate header for print version


Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12401*  Cite 
Description

Leishmania mexicana amastigotes in their parasitophorous vacuole in a mouse macrophage. Murine macrophages (cell line J774) were infected in vitro with Leishmania mexicana stationary phase promastigotes (WHO strain MNYC/BZ/62/M379). Four days post infection the cells were fixed with glutaraldehyde, post-fixed with osmium tetroxide, dehydrated in ethanol and embedded in epoxy resin. Thin sections (~90 nm) were viewed on a Philips Technai 12 transmission electron microscope operating at 80 kV. Magnification 2900X.

Biological Sources
NCBI Organism Classification
Leishmania mexicana
Mus musculus
Cell Type
protozoan parasites
macrophage
Cell Line
WHO strain MNYC/BZ/62/M379
J774
Cellular Component
parasite-containing vacuole
Biological Context
Biological Process
amastigote form Leishmania mexicana
parasite entry into host cell forming a parasitophorous vacuole
Attribution
Names
Eva Gluenz
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12401
Archival Resource Key (ARK)
ark:/b7295/w9cil12401
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 5112px 3.28nm
Y 4576px 3.28nm