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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12339*  Cite 

The AZM enters the oral cavity of Euplotes where it occupies the right side of the buccal cavity while cytopharyngeal lamellae composed of microtubules arise from the left wall of the cavity. Large vesicles that contain lipid-like osmiophilic material line up between the ribbons and make close contact with the single membrane of the food vacuole-forming cytopharynx. Under different fixation conditions these vesicles are compact and are very electron opaque. (See paper by Klotzel, J. Cell Sci. 15:379-401, 1974). Possible early fusion sites between the discoidal vesicles and the cytopharynx membrane are indicated by arrowheads. The elongated narrowed tips of the cilia extend into the nascent vacuole. TEM taken on 8/3/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Bar = 1µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. There is a high resolution version of this image in the library (CIL:39113) which is available for quantitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Euplotes sp.
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Euplotes sp. from Carolina Biological Supply
Cellular Component
oral apparatus
microtubule associated complex
Biological Context
Biological Process
oral apparatus organization
cytopharynx organization
cortical cytoskeleton organization
Richard Allen
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 2380px ——
Y 3000px ——