Alternate header for print version


Licensing
This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12329*  Cite 
Description

NIH3T3 cells showing the localization of nuclear transport factor RanBP1 and F-actin in interphase cells. Cells were fixed in formaldehyde and labeled with a primary antibody to RanBP1 and Cy3-conjugated secondary to (RanBP1), FITC-labelled phalloidin, and DAPI. Images were collected on a Zeiss LSM510 confocal using a scan speed 6, 8-line averaging and a 63X 1.4NA objective.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
NIH/3T3
Cellular Component
actin cytoskeleton
nucleus
nuclear transport factor RanBP1
Attribution
Names
Hicham Mahboubi
Ursula Stochaj
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12329
Archival Resource Key (ARK)
ark:/b7295/w9cil12329
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
4',6-diamidino-2-phenylindole (DAPI)
Fluorescein (FITC)
phalloidin
Cy3
primary antibody plus labeled secondary antibody
Processing History
color merge
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.8µm
Y 1024px 0.8µm
Channel Wavelength
1 DAPI, FITC, CY3nm