The Cell: An Image Library

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CIL:123^*

Description

Telomeres were isolated from chicken erythrocyte nuclei following restriction endonuclease treatment, and imaged by transmission EM after uranyl acetate negative staining. Like its DNA counterpart, telomeric chromatin has have a looped conformation, which likely aids in protection from the rapid degradation that occurs with free DNA ends.

Technical Details

The image was recorded with a FEI Tecnai 12 TEM at 100KV and recorded on a TVIPS 2048x2048 CCD with 2x binning. See Nikitina T, Woodcock CL. 2004. Closed chromatin loops at the ends of chromosomes. J Cell Biol.166:161-5.

Biological Sources

NCBI Organism Classification: Gallus gallus gallus
Cell Type: erythrocyte
Cellular Component: chromosome, telomeric region

Biological Context

Biological Process: chromatin assembly; chromosome organization
Molecular Function: structural constituent of chromatin

Attribution

Name: Chris Woodcock
Published: Nikitina T, Woodcock CL. 2004. Closed chromatin loops at the ends of chromosomes. J Cell Biol.166:161-5.
Pubmed: 2172301

Imaging

Image Type: charge coupled device (CCD)
Imaging Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Processing History: unprocessed raw image

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2048px	0.454nm
Y	2048px	0.454nm

*CIL – Cell Image Library accession number. Please use this to reference an image.