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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12294*  Cite 
Description

Localization of the microtubule anchoring factor LL5α (red), microtubules (green), and the microtubule plus-end binding protein, EB1 (blue) in an MCF-10Aeco epithelial cell. EB1 was concentrated in regions of LL5 accumulation. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 1 C from J Cell Biol (2010) 189 (5):901-917.

Technical Details

MCF-10Aeco cells were seeded on coverslips at a low density. At 3 h after seeding cells were fixed and immunostained for tubulin (Cy2, 488 nm laser), EB1 (rhodamine red-X, 543 nm laser) and LL5s (Cy5, 633 nm laser), and observed using a LSM510 confocal laser-scanning microscope system.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
MCF 10A
Cellular Component
microtubule
cell cortex part
Attribution
Names
Azusa Hotta
Tomomi Kawakatsu
Tomoya Nakatani
Toshitaka Sato
Chiyuki Matsui
Taiko Sukezane
Tsuyoshi Akagi
Tomoki Hamaji
Ilya Grigoriev
Anna Akhmanova
Yoshimi Takai
Yuko Mimori-Kiyosue
Published
J. Cell Biol. 2010. 189(5):901-917
Pubmed
PMID:20513769
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12294
Archival Resource Key (ARK)
ark:/b7295/w9cil12294
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
Cy2
Cy5
X-Rhodamine
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px ——
Y 1024px ——