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Description
Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP F802A . Endogenous Borealin is green and DAPI (DNA) is blue. See Supplementary Fig 2C panels 13-16 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
Biological Sources
- NCBI Organism Classification
- Gallus gallus gallus
- Cell Type
- memory B cell
- Cell Line
- DT40
- Cellular Component
- nuclear chromosome
- spindle
- chromosome, centromeric region
Biological Context
- Biological Process
- mitosis
Attribution
- Names
- Z. Xu
- H. Ogawa
- P. Vagnarelli
- J.H. Bergmann
- D.F. Hudson
- S. Ruchard
- T. Fukagawa
- W.C. Earnshaw
- K. Samejima
- Published
- Xu et al. 2009 J Cell Biol 187, 637-653
- Link
- http://jcb-dataviewer.rupress.org/jcb/...
- Pubmed
- 19951914
Grouping
This image is part of a group.Imaging
- Image Type
- charge coupled device (CCD)
- Imaging Mode
- widefield illumination
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- differences in adsorption or binding of stain
- Visualization Methods
- Fluorescein (FITC)
- TexasRed
- 4',6-diamidino-2-phenylindole (DAPI)
- Processing History
- constrained iterative deconvolution
- Data Qualifiers
- processed data
Sample Preparation
- Methods
- formaldehyde fixed tissue
- Relation To Intact Cell
- whole mounted tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 512px | —— |
| Y | 512px | —— |
| Z | 44 | —— |
*CIL – Cell Image Library accession number. Please use this to reference an image.