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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12101*  Cite 

Colpoda is a soil ciliate and this micrograph is characterized by numerous mucocysts and food vacuoles containing bacteria in various stages of digestion. This electron micrograph is near the posterior end of the cell and contains spongiome tubules of the contractile vacuole. Rows of cilia cover the cell and basal body/cilium complexes are present as dikinetids, two complexes in each cortical unit. TEM taken on 5/31/69 by R. Allen with Philips 300 operating at 60kV. Neg. 5,000X. Bar = 1µm. The negative was printed to paper and the image was scanned with a flatbed scanner and the 1µm bar added in Photoshop. This digitized image is available for qualitative analysis. A high resolution image is available in this library (CIL:38831) and can be used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (

Biological Sources
NCBI Organism Classification
Colpoda cucullus
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England # LB1615/1
Cellular Component
cell cortex
food vacuole
microtubule basal body
Biological Context
Biological Process
cytoplasmic organization
cellular response to food
Richard Allen
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Print from negative scanned to Photoshop.
Data Qualifiers
processed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 2689px ——
Y 3000px ——