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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12087*  Cite 
Description

Nascent Lamellipodia Emerge with Rough Edges and Rapidly Acquire a Smooth Morphology. A glass micropipette, acting as a barrier, was lowered into the path of movement of a migrating, coherent keratocyte overexpressing EGFP-VASP (an indicator of polymerizing actin). When the keratocyte reaches the barrier, the lamellipodium temporarily stops protruding forward, while EGFP-VASP becomes displaced from the edge of the region in contact with the barrier. When the micropipette barrier is removed (between 0:57 and 1:00), the leading edge of the lamellipodium immediately resumes protrusion and appears rough with several protruding microregions enriched in EGFP-VASP. Only 18 s after removal of the barrier, the keratocyte’s original shape and uniform EGFP-VASP localization at the leading edge are restored. Time ¼ min:s. Scale bar 25 micron. Corresponds to video S2 in PLos Biology 5:2035-2052, 2007.

Biological Sources
NCBI Organism Classification
Hypsophrys nicaraguensis
Cell Type
keratocyte
Cellular Component
VASP
lamellipodium
Attribution
Names
Catherine I. Lacayo
Zachary Pincus
Martijn M. VanDuijn
Cyrus A. Wilson
Daniel A. Fletcher
Frank B. Gertler
Alex Mogilner
Julie Theriot
Published
PLos Biology 5:2035-2052, 2007.
Pubmed
17760506
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12087
Archival Resource Key (ARK)
ark:/b7295/w9cil12087
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
phase contrast microscopy
fluorescence microscopy
Parameters Imaged
elastic scattering of photons
fluorescence emission
Source of Contrast
differences in amount of elastic light scattering
distribution of a specific protein
Visualization Methods
EGFP
visualization of contiguous regions
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 652px 0.118µm
Y 327px 0.118µm
Time 0.25 seconds