High resolution view of the quadrulus membranelle (4 rows of cilia) in the buccal cavity of Paramecium tetraurelia labelled for tubulin. Microtubules of the axonemes of the cilia and the triplet fibers of the basal bodies are labeled. Ribbons of microtubules arise from the side of the quadrulus next to the cytopharynx and single microtubules are liberally scattered about in this zone. The “sperm-shaped” structures are early endosomes with clathrin coated pits forming the "heads." TEM taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X.
Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a Nikon Coolscan 9000ED. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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