The Cell: An Image Library - Image CIL:11934

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CIL:11934^*

Description

Rat NRK stably expressing RFP targeted to mitochondrial matrix imaged at the M phase of the cell cycle. This image is a z-stack from a living cell collected on a Zeiss 510 LSM confocal using a 63X 1.4NA objective. There is a related z-series of a cell in G1/S transition. The stable mito-RFP expressing NRK cell line was prepared by Kasturi Mitra.

Biological Sources

NCBI Organism Classification: Rattus; (Old World rats)
Cell Line: NRK; Kidney
Cellular Component: mitochondrion

Biological Context

Biological Process: M phase of mitotic cell cycle

Attribution

Name: Brenda Kostelecky

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: mRFP1
Processing History: unprocessed raw data

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.093μm
Y	512px	0.093μm
Z	23	1μm

*CIL – Cell Image Library accession number. Please use this to reference an image.