Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11811^* Cite

Description

Short timelapse movie of prechordal plate, hypoblast, and yolk syncytial layer (YSL) nuclear movements during 80-90% epiboly. The prechordal plate and notochord can be seen as a tighter clump of cells at the dorsal midline while the deeper YSL nuclei are the large bright spots. Cell movement in the hypoblast is quite dynamic. Alexa-Histone injected into YSL, free Alexa permeates to label deep cells in blastoderm. Sphere is approximately 700 microns in diameter. Video made available through Mark Cooper and Zebrafish The Living Laboratory.

Biological Sources

NCBI Organism Classification: Danio rerio
Cell Type: yolk cell
Cellular Component: nucleus

Biological Context

Biological Process: cell migration; embryo development
Zebrafish Anatomy: prechordal plate; notochord; hypoblast

Attribution

Names: Leonard A. D'Amico; Mark S. Cooper
Link: fishscope
OTHER: Zebrafish The Living Laboratory

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11811
Archival Resource Key (ARK): ark:/b7295/w9cil11811

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; differential interference contrast microscopy
Parameters Imaged: optical path length gradient; fluorescence emission
Source of Contrast: boundaries between regions with different refractive index; differences in adsorption or binding of stain
Visualization Methods: Alexa-Histone
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	622px	——
Y	512px	——