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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11560*  Cite 
Description

Figs. 305 & 306 from Don Fawcett's Chapter 12: Centrioles show a cross section though centrioles. The radially symmetrical periodic structures were intensified by rotating and averaging the images. The cross section of a centriole at the upper left has been intensified by summation of fractional exposures through nine steps of rotation to produce the image at the upper right. This accentuates the hub-and-spokes pattern of densities in the interior of the centriole. The cross section of a centriole stained with tannic acid in the lower figure shows the protofilaments of the triplets in negative image. Like other microtubules, the wall of subunit, which is the innermost ring of the triplets, a has 13 tubulin protofilaments, while the outer two rings each have 10. Although the dimensions of the hub are similar to those of a microtubule, no protofilaments are detectable in its wall. Please see CID 11558 for further description.

Biological Sources
NCBI Organism Classification
unknown eukaryote
Cell Type
eukaryotic cell
Cellular Component
centriole
microtubule
Biological Context
Biological Process
centriole organization
cytoplasmic microtubule organization
Attribution
Names
Don W. Fawcett
Etienne de Harven
Vitauts Kalnins
Published
ISBN 0721635849
Link
Fawcett The Cell
Chapter 12 PDF
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11560
Archival Resource Key (ARK)
ark:/b7295/w9cil11560
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 887px ——
Y 1272px ——