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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11166*  Cite 
Description

The remarkable soccer ball-like structure of coated vesicles is revealed through negative staining, and the inset panel shows a candidate model of the lattice structure. Coating of vesicles is important for shaping, packaging, sorting, and transporting the vesicle and its contents. In this preparation, coated vesicles were isolated from homogenized guinea pig brain tissue. Both upper and lower panels labeled A, B are from Kanaseki and Kadota (1969) J. Cell Biol. 42:202-220, reprinted with permission in Chapter 2 (Specializations of the Free Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. as Figure 59 (upper panels, magnification, 300,000X), and Fig 60 A & B (magnification, 400,000X). Fig. 60 C & D are courtesy of T. Roth, and show the same image before (C) and after (D) photographic enhancement using Markam rotation. A PDF copy of the accompanying chapter is available on the ASCB’s BioEDUCATE website.

Biological Sources
NCBI Organism Classification
Cavia porcellus
Cellular Component
clathrin vesicle coat
Biological Context
Biological Process
regulation of endocytosis
Attribution
Names
Don W. Fawcett
Toku Kanaseki
Ken Kadota
Thomas Roth
Published
ISBN 0721635849
Pubmed
PMID: 4182372
Link
Fawcett's "The Cell, 2nd Ed."
Kanaseki & Kadota (1969) J. Cell Biol.
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11166
Archival Resource Key (ARK)
ark:/b7295/w9cil11166
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Visualization Methods
osmium tetroxide
uranyl salt
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 974px ——
Y 1252px ——