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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10709*  Cite 
Description

Meiotic newt oocyte nuclei were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 10 KX. This micrograph, showing transcription on looped lampbrush chromosomes was recorded at a tilt angle of 45 degrees. The image is grouped with one of the same sample area recorded at a 55 degree tilt. A stereo examination of the pair provides an oblique 3D view.

Biological Sources
NCBI Organism Classification
Notophthalmus viridescens
Cell Type
oocyte
Cellular Component
lampbrush chromosomes
Biological Context
Biological Process
Transcription
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10709
Archival Resource Key (ARK)
ark:/b7295/w9cil10709
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
film
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 5065px 1.75µm
Y 3608px 1.75µm