A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. Corresponds to deconvolved stack: CIL# 10539, maximum intensity projection stacks: CIL# 10540 and CIL#1 10541 and raw data stack with DIC: CIL#10543. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.
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