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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10525*  Cite 

Time-lapse of Toxoplasma gondii infection of human foreskin fibroblast cells. T. gondii stably express EGFP-tagged TgIMC1 and mRFP-tagged TgMORN1 (MORN domain containing protein). Formation of daughter cells is visible during time-lapse. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. 3D stacks of images were collected at 0.6 μ increments in z, every 10 min. Maximum intensity projection of 3D deconvolved stacks at each timepoint were used to generate mpg movie. Corresponds to raw data: CIL# 10525. and 3D deconvolved data: CIL# 10523 and projection of 3D deconvolved data: CIL# 10524. Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details.

Biological Sources
NCBI Organism Classification
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH stable cell line
Cellular Component
inner membrane complex
spindle pole
basal part of cell
Biological Context
Biological Process
regulation of cell shape
cytoskeleton organization
cell division
Human Disease
John Murray (University of Pennsylvania)
Ke Hu (Indiana University, Bloomington)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
charge coupled device (CCD)
Image Mode
fluorescence microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 576px ——
Y 416px ——