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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10522*  Cite 

Time-lapse of Toxoplasma gondii. A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgIMC1 and mRFP-tagged TgMORN1 (MORN domain containing protein). Formation of daughter cells is visible during time-lapse. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. The nonfluorescent host fibroblasts are seen in the DIC image only. 3D stacks of images were collected at 0.6 μ increments in z, every 10 min. The DIC image is a single plane from the middle of the stack. Corresponding deconvolved stack: CIL# 10523 and projection of the deconvolved stack: CIL# 10524 and movie of projection: CIL# 10525 Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details.

Biological Sources
NCBI Organism Classification
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH stable cell line
Cellular Component
inner membrane complex
spindle pole
basal part of cell
Biological Context
Biological Process
regulation of cell shape
cytoskeleton organization
cell division
Human Disease
John Murray (University of Pennsylvania)
Ke Hu (Indiana University, Bloomington)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 256px 0.2134µm
Y 256px 0.2134µm
Z 9px 0.6µm
Time 600 seconds 100