Time-lapse of Toxoplasma gondii. A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgIMC1 and mRFP-tagged TgMORN1 (MORN domain containing protein). Formation of daughter cells is visible during time-lapse. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. The nonfluorescent host fibroblasts are seen in the DIC image only. 3D stacks of images were collected at 0.6 μ increments in z, every 10 min. The DIC image is a single plane from the middle of the stack. Corresponding deconvolved stack: CIL# 10523 and projection of the deconvolved stack: CIL# 10524 and movie of projection: CIL# 10525 Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details.
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