The Cell: An Image Library - Image CIL:10521

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CIL:10521^*

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgMORN1 and mCherryFP-tagged Tgalpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Captured with CoolSnapHQ/ICX285. Deconvolved stack corresponds to raw data stack: CIL# 10520. Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: cortical microtubule; centriole; spindle pole; conoid; basal part of cell

Biological Context

Biological Process: regulation of cell shape; microtubule cytoskeleton organization
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Name: Ke Hu (Indiana University, Bloomington)

Grouping

This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Imaging Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; mCherryFP
Processing History: 3D-deconvolution

Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	128px	.1326μm
Y	128px	.1326μm
Z	11	.4μm

*CIL – Cell Image Library accession number. Please use this to reference an image.