The Cell: An Image Library - Image CIL:10485

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CIL:10485^*

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: GFP, ex 450-490 em 500-540. Captured with CoolSnapHQ/ICX285. Stack corresponds to deconcolved data stack: CIL# 10486. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: cortical microtubule

Biological Context

Biological Process: regulation of cell shape; microtubule cytoskeleton organization
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Name: John Murray (University of Pennsylvania)

Grouping

This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Imaging Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	256px	.1067μm
Y	256px	.1067μm
Z	13	.3μm

*CIL – Cell Image Library accession number. Please use this to reference an image.