The Cell: An Image Library - Image CIL:10468

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CIL:10468^*

Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin. Images overlaid with DIC. Images acquired using API DeltaVision on an Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: GFP, ex 450-490 em 500-540. Captured with CoolSnapHQ/ICX285. 3D stack is raw data corresponding to deconvolved stack: CIL# 10469. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: cortical microtubule

Biological Context

Biological Process: regulation of cell shape; microtubule cytoskeleton organization
Molecular Function: structural constituent of cytoskeleton
Human Disease: toxoplasmosis

Attribution

Name: John Murray (University of Pennsylvania)

Grouping

This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Imaging Mode: fluorescence microscopy; differential interference contrast microscopy
Parameters Imaged: fluorescence emission; optical path length gradient
Source of Contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization Methods: EGFP

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	.1067μm
Y	1024px	.1067μm
Z	12	.3μm

*CIL – Cell Image Library accession number. Please use this to reference an image.