The Cell: An Image Library - Image CIL:10465

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CIL:10465^*

Description

Toxoplasma gondii expressing EGFP tagged Tgbeta2-tubulin and mCherry-TgDCCP (Double cortin containing protein), an unpublished microtubule binding protein. Images overlaid with DIC. For GFP, ex 450-490 em 500-540; for mCherry ex 555-592 em 600-665. Images acquired using API Delta Vision on a Olympus ix70 wide-field microscope. Objective: Olympus 60X water immersion, 1.2 NA. Captured with CoolSnapHQ/ICX285. 23 images in z-series; this is raw,unprocessed data stack; 3D deconvolved stack is: CIL# 10466. Image is a control not treated with oryzalin. Part of an experiment with some oryzalin-treatment. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: cortical microtubule

Biological Context

Biological Process: regulation of cell shape
Molecular Function: structural constituent of cytoskeleton

Attribution

Name: John Murray (University of Pennsylvania)

Grouping

This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Imaging Mode: fluorescence microscopy; differential interference contrast microscopy
Parameters Imaged: fluorescence emission; optical path length gradient
Source of Contrast: distribution of a specific protein; boundaries between regions with different refractive index
Visualization Methods: EGFP; mCherryFP

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	256px	.1067μm
Y	256px	.1067μm
Z	23	.3μm

*CIL – Cell Image Library accession number. Please use this to reference an image.