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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10458*  Cite 

Toxoplasma gondii expressing EGFP tagged Tgbeta1-tubulin and TgAPR1-mCherry (TgAPR1 is a Microtubule Organizing Center protein). For GFP, ex 450-490 em 500-540; for mCherry ex 555-592 em 600-665. Images acquired using API DeltaVision on a Olympus ix70 wide-field microscope. Objective: Olympus 60X water immersion, 1.2 NA. Captured with CoolSnapHQ/ICX285. 15 images in z-series; this is the deconvolved 3D stack corresponding to raw data CIL# 10458 and stack with DIC CIL# 10461. Unpublished image similar to images published in PMID: 16518471. Also see publication for experimental details.

Biological Sources
NCBI Organism Classification
Toxoplasma gondii RH
Cell Type
Toxoplasma gondii RH tachyzoite
Cell Line
Toxoplasma gondii RH double stable clone expressing TgAPR1-mCherry and EGFP-beta1-tubulin
Cellular Component
cortical microtubule cytoskeleton
microtubule organizing center
Biological Context
Biological Process
regulation of cell shape
Molecular Function
structural constituent of cytoskeleton
John Murray
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
charge coupled device (CCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 512px 106.7nm
Y 512px 106.7nm
Z 15px 300nm