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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10426*  Cite 

Nucleated erythrocytes from chicken were spread on water, the dispersed chromatin picked up on carbon-formvar grids, fixed with paraformaldehye, critical point dried and shadowed with platinum. Images were obtained with the Wisconsin high voltage TEM at 1MEV using darkfield illumination. For this micrograph, the grid was tilted to 55 degrees. A similar micrograph tilted to 44 degrees providing a stereo pair with an oblique 3D view of the tangled and irregular chromatin fibers is grouped with this image. The combination of shadowing and darkfield illumination gives a quasi-negative stain effect revealing nucleosomes in some fibers.

Biological Sources
NCBI Organism Classification
Gallus gallus
Cell Type
Cellular Component
nuclear chromatin
Hans Ris
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
transmission electron microscopy (TEM)
darkfield illumination
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Processing History
unprocessed raw data
Sample Preparation
formaldehyde fixed tissue
critical_point dried specimen
Relation To Intact Cell
spread preparation
Spatial Axis Image Size Pixel Size
X 2566px 0.5nm
Y 2742px 0.5nm