Licensing

Attribution_nc_sa
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Description

Projection of a HeLa cell expressing a YFP-SC35 fusion protein that localizes to nuclear speckles and in a diffuse nuclear localization. Image stack was acquired using a Deltavision deconvolution microscope.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
cervical carcinoma
Cell Line
HeLa
Cellular Component
nucleus

Biological Context

Biological Process
RNA splicing
RNA metabolic process

Attribution

Names
R.I. Kumaran
Z. Lazar
David L. Spector

Imaging

Image Type
recorded image
Imaging Mode
fluorescence microscopy
constrained iterative deconvolution
charge coupled device (CCD)
Parameters Imaged
fluorescence emission
Source of Contrast
level of fluorochrome
Visualization Methods
EYFP
Processing History
constrained iterative deconvolution
projection of image stack
Application of green LUT

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 857px 40nm
Y 1071px 40nm
*CIL – Cell Image Library accession number. Please use this to reference an image.