The Cell: An Image Library - Image CIL:10017

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CIL:10017^*

Description

Organization of Golgi and endoplasmic reticulum (ER) in a Human HeLa cell expressing Sialyl Transferase-EGFP (green) and Invariant Chain33-mRFP (red). The Sialyl Transferase-EGFP is a Golgi marker and the Invariant Chain33-mRFP is retained in the ER. There are two related files for this specimen: the z series and the maximum projection. This file is the maximum projection. Zeiss Spinning Disc confocal used with with 220 ms exposure and a 63X oil objective.

Biological Sources

NCBI Organism Classification: Homo sapiens; (human)
Cell Type: permanent cell line cell; epithelial cell
Cell Line: HeLa
Cellular Component: Golgi apparatus; endoplasmic reticulum

Biological Context

Biological Process: Golgi organization
Molecular Function: endoplasmic reticulum organization

Attribution

Name: Prabuddha Sengupta

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: mRFP1
Processing History: maximum projection

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.110μm
Y	512px	0.110μm

*CIL – Cell Image Library accession number. Please use this to reference an image.