Licensing
Description
The locomotor cortex of Didinium. A longitudinal section of basal bodies and the axonemes of cilia is revealed as they emerge from the cell cortex of Didinium. Bundles of microtubules extend from the basal bodies into the endoplasm through the fibrous layer that characterizes the cortex of Didinium. Tubular or flattened early endosomes with coated pits at their margins are present in the endoplasm beneath the fibrous layer. Mitochondrial cristae appear to be decorated by F1F0 ATP synthases. Cross sections of three bacteria are noted between the cilia. TEM taken on 5/9/69 by M. Sage with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:4661) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Didinium nasutum
- Cell Type
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cell Line
- Carolina Biological Supply Company, NC, U.S.A.
- Cellular Component
- cell cortex
- cilium
- microtubule basal body
- ciliary rootlet
- mitochondrion
Biological Context
- Biological Process
- ciliary cell motility
- microtubule cytoskeleton organization
- Molecular Function
- cytoskeletal protein binding
Attribution
- Names
- M. Sage
- Richard Allen
- Link
- http://www5.pbrc.hawaii.edu/allen/
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- detection of electrons
- film
- Parameters Imaged
- electron density
- Source of Contrast
- stain with broad specificity
- Visualization Methods
- stain with broad specificity
- osmium tetroxide
- uranyl salt
- lead salt
- Processing History
- film
- Print from negative scanned for Photoshop.
- Data Qualifiers
- processed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- microtome-sectioned tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 3496px | —— |
| Y | 2792px | —— |