Licensing
Description
Didinium has two bands of ciliated basal bodies that encircle the cell, one adorally and one aborally. The bands are composed of groups of approximately 15 ciliated basal bodies. This section is a longitudinal section of the cilium and basal body and cross section of approximately seven different groups or pectinelles. The proximal end of basal bodies are ensheathed in electron opaque sleeves from which microtubular ribbons extend into the adjacent ridges on the two sides of each basal body. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X.Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:4664) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Didinium nasutum
- Cell Type
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cell Line
- Carolina Biological Supply Company, NC, U.S.A.
- Cellular Component
- cell cortex
- cilium
- microtubule basal body
- ciliary rootlet
- axoneme
- ectosymbiotic bacteria
Biological Context
Attribution
- Name
- Richard Allen
- Link
- http://www5.pbrc.hawaii.edu/allen/
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- detection of electrons
- film
- Parameters Imaged
- electron density
- Source of Contrast
- stain with broad specificity
- Visualization Methods
- stain with broad specificity
- osmium tetroxide
- uranyl salt
- lead salt
- Processing History
- film
- Print from negative scanned for Photoshop.
- Data Qualifiers
- processed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- microtome-sectioned tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 3400px | —— |
| Y | 2725px | —— |