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Center for Research in Biological Systems
Basic Science Building, Room 1000
University of California, San Diego
9500 Gilman Drive
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Grouped images - the images shown below are related
CIL:13862
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cytokinesis
Cellular Component
spindle pole body
In tem1Δ cells expressing a Cnm67-Tem1 fusion protein, Cdc15-3HA (red) loads prematurely onto spindle pole bodies during metaphase as determined by spindle morphology (tubulin, green) and nuclear mor...
CIL:13863
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cytokinesis
Cellular Component
spindle pole body
In tem1Δ cells expressing a Bfa1-Tem1 fusion protein, Cdc15-3HA (red) loads prematurely onto spindle pole bodies during metaphase as determined by spindle morphology (tubulin, green) and nuclear morp...
CIL:13864
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cytokinesis
Cellular Component
spindle pole body
Cdc15-3HA (red) does not load onto spindle pole bodies in wild-type cells in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control image for CIL# ...
CIL:13865
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
spindle pole body
3HA-Cdc14 (red) localization in anaphase. The protein is released throughout the whole cell. Anaphase is determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Image is...
CIL:13866
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
spindle pole body
3HA-Cdc14 (red) localization in early anaphase. The protein is partially released throughout the nucleus. Early anaphase is determined by spindle morphology (tubulin, green) and nuclear morphology (DA...
CIL:13867
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
spindle pole body
3HA-Cdc14 (red) localization in metaphase. The protein is sequestered in the nucleolus. Metaphase is determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Image is Fig...
CIL:13868
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
During late anaphase, Tem1 (green) localized to both spindle pole bodies (SPBs), but Bfa1 (red) was asymmetrically localized to the daughter SPB. DAPI (blue) and DIC (hidden) are also shown. Compare t...
CIL:13869
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
Both Tem1 (green) and Bfa1 (red) are localized to both spindle pole bodies in metaphase. DAPI (blue) and DIC (hidden) are also shown. Compare to anaphase image CIL# 13868. Image is Fig 6A, top panels,...
CIL:13870
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
Tem1 (green) loading onto spindle pole bodies during anaphase is severely affected in bfa1Δ cells. Compare with control image CIL# 13871. DAPI (blue) and DIC (hidden) are also shown. Image is Fig 6B,...
CIL:13871
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Tem1 (green) primarily localizes to the daughter cell spindle pole body (SPB) during anaphase. Control image for CIL# 13870 in which Tem1 loading onto SPBs was severely affected in bfa1Δ cells. DAPI ...
CIL:13872
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
cytoskeleton
Tem1-CAAX (green) is targeted away from spindle pole bodies in bfa1Δ cells. The CAAX motif from Ras2 targets proteins to the cytoplasmic side of the membrane. tem1Δ::GAL-UPL-TEM1 cells expressing eG...
CIL:13873
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
cytoskeleton
Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows an aligned spindle, CIL #1...
CIL:13874
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
cytoskeleton
Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a rebudded cell, CIL #1387...
CIL:13875
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
3HA-Bfa1 (red) localizes to both spindle pole bodies (SPBs) in anaphase in dyn1Δ cells with constitutive targeting of Tem1 to both SPBs. However, the constitutive presence of Tem1 on the SPBs impaire...
CIL:13876
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
Tem1 (green) loading onto spindle pole bodies during metaphase is severely affected in bfa1Δ cells. Compare with control image CIL# 13877. DAPI (blue) and DIC (hidden) are also shown. CIL# 13871 and ...
CIL:13877
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Tem1 (green) localizes to spindle pole bodies (SPB) during metaphase. Control image for CIL# 13876 in which Tem1 loading onto SPBs was severely affected in bfa1∆ cells. DAPI (blue) and DIC (hidden) ...
CIL:13878
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cytokinesis
Cellular Component
spindle pole body
During late anaphase, Tem1 (green) localized to the spindle pole body (SPB) in cdc15-2 bfa1Δ cells grown at the restrictive temperature. DAPI (blue) and DIC (hidden) are also shown. Compare to image ...
CIL:13879
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cytokinesis
Cellular Component
spindle pole body
During late anaphase, Tem1 (green) localized to the spindle pole body (SPB) in cdc15-2 cells grown at the restrictive temperature. DAPI (blue) and DIC (hidden) are also shown. Compare to image CIL# 13...
CIL:13880
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
spindle pole body
Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (this image) but can also still localize to the SPB (CIL# 13881). The CAAX motif from Ras2 targets proteins to the cytopl...
CIL:13881
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
spindle pole body
Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (CIL# 13880) but can also still localize to the SPB (this image). The CAAX motif from Ras2 targets proteins to the cytopl...
CIL:13882
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13885). This image shows SPB localization of eGFP-Tem1 (eGFP, g...
CIL:13883
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (this image) and anaphase cells (CIL#13886). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-CNM67–TEM1 from a CEN plasmid...
CIL:13884
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (this image) and anaphase cells (CIL#13887). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-BFA1–TEM1 from a CEN plasmid ...
CIL:13885
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13882). This image shows SPB localization of eGFP-Tem1 (eGFP, g...
CIL:13886
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (CIL# 13883) and anaphase cells (this image). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-CNM67–TEM1 from a CEN plasmi...
CIL:13887
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (CIL# 13884) and anaphase cells (this image). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-BFA1–TEM1 from a CEN plasmid...
CIL:13888
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
cytoskeleton
Tem1 (green) normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase; however, Tem1's ability to localize to the SPB is severely decreased in bfa...
CIL:13889
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
cytoskeleton
Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a misaligned spindle, CIL ...
CIL:13890
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
3HA-Bfa1 (red) is generally localized to one spindle pole body (SPB) in wild-type cells in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control i...
CIL:13891
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
3HA-Bfa1 (red) is now symmetrically localized to both spindle pole bodies (SPB) in cells with constitutive targeting of Tem1 to both SPBs in metaphase as determined by spindle morphology (tubulin, gre...
CIL:13892
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
3HA-Bfa1 (red) is generally localized to the daughter spindle pole body (SPB) in wild-type cells in anaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). C...
CIL:13893
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
3HA-Bfa1 (red) is now symmetrically localized to both spindle pole bodies (SPB) in cells with constitutive targeting of Tem1 to both SPBs in anaphase as determined by spindle morphology (tubulin, gree...
CIL:13894
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
mitotic cell cycle spindle orientation checkpoint
Cellular Component
spindle pole body
DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with galactose. Overexpression of Kin4 arrests cells in anaphase. Compare with control image CIL# 13895. Image is Fig...
CIL:13895
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
mitotic cell cycle spindle orientation checkpoint
Cellular Component
spindle pole body
DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with glucose. This is the control image for CIL# 13894 in which overexpression of Kin4 arrests cells in anaphase. Ima...
CIL:13896
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
mitotic cell cycle spindle orientation checkpoint
Cellular Component
spindle pole body
DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of T...
CIL:13897
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
mitotic cell cycle spindle orientation checkpoint
Cellular Component
spindle pole body
DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with glucose. This is the control image f...
